Review



cell membrane permeable calcium fluorescent probe fluo 3 am  (Yeasen Biotechnology)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Yeasen Biotechnology cell membrane permeable calcium fluorescent probe fluo 3 am
    A , B SH-SY5Y cells were transfected with an empty pCRIPERV2 vector (NC) or pCRIPERV2-gRNA plasmids (Mettl14-KD#1 and Mettl14-KD#2), 12 h later, cellular Ca 2+ was detected <t>by</t> <t>FLUO-3</t> AM probe and flow cytometry. C SH-SY5Y cells were treated the same as ( A ), 12 h later, cellular Ca 2+ was observed under microscope (bar = 10 μm). D The endoplasmic reticulum and mitochondria of neurons in SN of Ctrl and Mettl14-cKO mice were observed under a transmission electron microscope (bar = 1 μm left or 500 nm right). E , F The distance between ER and outer mitochondrial membrane (OMM) and the number of ER-Mito contacts per 10 μm Mitochondrial membrane surface were analyzed ( n = 4). G SH-SY5Y cells were treated the same as ( A ), 12 or 24 h later, ER stress marker proteins GRP78, ATF4 and CHOP expression were detected by WB assay. H – J Quantification of WB. All data are presented as mean ± SD, ns indicated no significant, * P < 0.05, ** P < 0.01 compared to NC.
    Cell Membrane Permeable Calcium Fluorescent Probe Fluo 3 Am, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+membrane+permeable+calcium+fluorescent+probe+fluo+3+am/2+am+rhod/pmc13144520-314-0-12
    Average 86 stars, based on 1 article reviews
    cell membrane permeable calcium fluorescent probe fluo 3 am - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Loss of METTL14 in dopaminergic neurons disrupts ER homeostasis via m6A-dependent regulation of Atp2a3 mRNA: Implications for Parkinson’s Disease"

    Article Title: Loss of METTL14 in dopaminergic neurons disrupts ER homeostasis via m6A-dependent regulation of Atp2a3 mRNA: Implications for Parkinson’s Disease

    Journal: NPJ Parkinson's Disease

    doi: 10.1038/s41531-026-01318-7

    A , B SH-SY5Y cells were transfected with an empty pCRIPERV2 vector (NC) or pCRIPERV2-gRNA plasmids (Mettl14-KD#1 and Mettl14-KD#2), 12 h later, cellular Ca 2+ was detected by FLUO-3 AM probe and flow cytometry. C SH-SY5Y cells were treated the same as ( A ), 12 h later, cellular Ca 2+ was observed under microscope (bar = 10 μm). D The endoplasmic reticulum and mitochondria of neurons in SN of Ctrl and Mettl14-cKO mice were observed under a transmission electron microscope (bar = 1 μm left or 500 nm right). E , F The distance between ER and outer mitochondrial membrane (OMM) and the number of ER-Mito contacts per 10 μm Mitochondrial membrane surface were analyzed ( n = 4). G SH-SY5Y cells were treated the same as ( A ), 12 or 24 h later, ER stress marker proteins GRP78, ATF4 and CHOP expression were detected by WB assay. H – J Quantification of WB. All data are presented as mean ± SD, ns indicated no significant, * P < 0.05, ** P < 0.01 compared to NC.
    Figure Legend Snippet: A , B SH-SY5Y cells were transfected with an empty pCRIPERV2 vector (NC) or pCRIPERV2-gRNA plasmids (Mettl14-KD#1 and Mettl14-KD#2), 12 h later, cellular Ca 2+ was detected by FLUO-3 AM probe and flow cytometry. C SH-SY5Y cells were treated the same as ( A ), 12 h later, cellular Ca 2+ was observed under microscope (bar = 10 μm). D The endoplasmic reticulum and mitochondria of neurons in SN of Ctrl and Mettl14-cKO mice were observed under a transmission electron microscope (bar = 1 μm left or 500 nm right). E , F The distance between ER and outer mitochondrial membrane (OMM) and the number of ER-Mito contacts per 10 μm Mitochondrial membrane surface were analyzed ( n = 4). G SH-SY5Y cells were treated the same as ( A ), 12 or 24 h later, ER stress marker proteins GRP78, ATF4 and CHOP expression were detected by WB assay. H – J Quantification of WB. All data are presented as mean ± SD, ns indicated no significant, * P < 0.05, ** P < 0.01 compared to NC.

    Techniques Used: Transfection, Plasmid Preparation, Flow Cytometry, Microscopy, Transmission Assay, Membrane, Marker, Expressing

    A , B SH-SY5Y cells were transfected with pCRIPERV2-gRNA#1 plasmid (Mettl14-KD) or pCMV-Atp2a3-cDNA coding plasmid (Atp2a3-OE), combined with their empty vectors (Control: cells were transfected with empty pCRIPERV2 and empty pCMV vectors; Mettl14-KD: cells were transfected with pCRIPERV2-gRNA#1 and empty pCMV vector; KD&Atp2a3-OE: cells were transfected with pCRIPERV2-gRNA#1 and pCMV-Atp2a3-cDNA coding plasmid). Twelve hours later, cellular Ca 2+ was detected by FLUO-3 AM probe and flow cytometry. C SH-SY5Y cells were treated the same as ( A ), 12 h later, ER stress marker proteins GRP78, ATF4 and CHOP expression were detected by WB assay. D – F Quantification of WB. G , H SH-SY5Y cells were treated the same as ( A ), 12 h later, cells were treated DCFH-DA for 15 min and the cellular ROS was detected by flowcytometry. The mean DCF fluorescence intensity were quantified as relative DCFH-DA DCFH-DA level. I SH-SY5Y cells were treated the same as ( A ), 24 h later, relative cell number was quantified by cell counting assay. All data are presented as mean ± SD. * P < 0.05, ** P < 0.01 compared to Control; ## P < 0.01 compared to Mettl14-KD.
    Figure Legend Snippet: A , B SH-SY5Y cells were transfected with pCRIPERV2-gRNA#1 plasmid (Mettl14-KD) or pCMV-Atp2a3-cDNA coding plasmid (Atp2a3-OE), combined with their empty vectors (Control: cells were transfected with empty pCRIPERV2 and empty pCMV vectors; Mettl14-KD: cells were transfected with pCRIPERV2-gRNA#1 and empty pCMV vector; KD&Atp2a3-OE: cells were transfected with pCRIPERV2-gRNA#1 and pCMV-Atp2a3-cDNA coding plasmid). Twelve hours later, cellular Ca 2+ was detected by FLUO-3 AM probe and flow cytometry. C SH-SY5Y cells were treated the same as ( A ), 12 h later, ER stress marker proteins GRP78, ATF4 and CHOP expression were detected by WB assay. D – F Quantification of WB. G , H SH-SY5Y cells were treated the same as ( A ), 12 h later, cells were treated DCFH-DA for 15 min and the cellular ROS was detected by flowcytometry. The mean DCF fluorescence intensity were quantified as relative DCFH-DA DCFH-DA level. I SH-SY5Y cells were treated the same as ( A ), 24 h later, relative cell number was quantified by cell counting assay. All data are presented as mean ± SD. * P < 0.05, ** P < 0.01 compared to Control; ## P < 0.01 compared to Mettl14-KD.

    Techniques Used: Transfection, Plasmid Preparation, Control, Flow Cytometry, Marker, Expressing, Fluorescence, Cell Counting

    Related Articles

    Membrane:

    Article Title: Loss of METTL14 in dopaminergic neurons disrupts ER homeostasis via m6A-dependent regulation of Atp2a3 mRNA: Implications for Parkinson’s Disease
    Article Snippet: Alexa fluor 594-conjugated goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody (A11012), Alexa fluor 488-conjugated goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody (A11008), Alexa fluor 594-conjugated goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody (A11005) and Alexa fluor 488-conjugated goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody (A11001) were from Thermo Fisher Scientific. .. Cell membrane permeable calcium fluorescent probe Fluo-3 AM (40703ES50) was purchased from Yeasen Biotechnology. .. DCFH-DA (2′,7′-dichlorodihydrofluorescein diacetate, CA1410) for reactive oxygen species detection was obtained from Solarbio life Sciences.

    Article Title: Loss of METTL14 in dopaminergic neurons disrupts ER homeostasis via m6A-dependent regulation of Atp2a3 mRNA: Implications for Parkinson's Disease.
    Article Snippet: Alexa fluor 594‐ conjugated goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (A11012), Alexa fluor 488‐ conjugated goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (A11008), Alexa fluor 594‐ conjugated goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody (A11005) and Alexa fluor 488‐ conjugated goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody (A11001) were from Thermo Fisher Scientific. .. Cell membrane permeable calcium fluorescent probe Fluo-3 AM (40703ES50) was purchased from Yeasen Biotechnology. .. DCFH-DA (2′,7′-dichlorodihydrofluorescein diacetate, CA1410) for reactive oxygen species detection was obtained from Solarbio life Sciences.



    Similar Products

    cell membrane permeable calcium fluorescent probe fluo 3 am  (Yeasen Biotechnology)
    86
    Yeasen Biotechnology cell membrane permeable calcium fluorescent probe fluo 3 am
    A , B SH-SY5Y cells were transfected with an empty pCRIPERV2 vector (NC) or pCRIPERV2-gRNA plasmids (Mettl14-KD#1 and Mettl14-KD#2), 12 h later, cellular Ca 2+ was detected <t>by</t> <t>FLUO-3</t> AM probe and flow cytometry. C SH-SY5Y cells were treated the same as ( A ), 12 h later, cellular Ca 2+ was observed under microscope (bar = 10 μm). D The endoplasmic reticulum and mitochondria of neurons in SN of Ctrl and Mettl14-cKO mice were observed under a transmission electron microscope (bar = 1 μm left or 500 nm right). E , F The distance between ER and outer mitochondrial membrane (OMM) and the number of ER-Mito contacts per 10 μm Mitochondrial membrane surface were analyzed ( n = 4). G SH-SY5Y cells were treated the same as ( A ), 12 or 24 h later, ER stress marker proteins GRP78, ATF4 and CHOP expression were detected by WB assay. H – J Quantification of WB. All data are presented as mean ± SD, ns indicated no significant, * P < 0.05, ** P < 0.01 compared to NC.
    Cell Membrane Permeable Calcium Fluorescent Probe Fluo 3 Am, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+membrane+permeable+calcium+fluorescent+probe+fluo+3+am/2+am+rhod/pmc13144520-314-0-12
    Average 86 stars, based on 1 article reviews
    cell membrane permeable calcium fluorescent probe fluo 3 am - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    A , B SH-SY5Y cells were transfected with an empty pCRIPERV2 vector (NC) or pCRIPERV2-gRNA plasmids (Mettl14-KD#1 and Mettl14-KD#2), 12 h later, cellular Ca 2+ was detected by FLUO-3 AM probe and flow cytometry. C SH-SY5Y cells were treated the same as ( A ), 12 h later, cellular Ca 2+ was observed under microscope (bar = 10 μm). D The endoplasmic reticulum and mitochondria of neurons in SN of Ctrl and Mettl14-cKO mice were observed under a transmission electron microscope (bar = 1 μm left or 500 nm right). E , F The distance between ER and outer mitochondrial membrane (OMM) and the number of ER-Mito contacts per 10 μm Mitochondrial membrane surface were analyzed ( n = 4). G SH-SY5Y cells were treated the same as ( A ), 12 or 24 h later, ER stress marker proteins GRP78, ATF4 and CHOP expression were detected by WB assay. H – J Quantification of WB. All data are presented as mean ± SD, ns indicated no significant, * P < 0.05, ** P < 0.01 compared to NC.

    Journal: NPJ Parkinson's Disease

    Article Title: Loss of METTL14 in dopaminergic neurons disrupts ER homeostasis via m6A-dependent regulation of Atp2a3 mRNA: Implications for Parkinson’s Disease

    doi: 10.1038/s41531-026-01318-7

    Figure Lengend Snippet: A , B SH-SY5Y cells were transfected with an empty pCRIPERV2 vector (NC) or pCRIPERV2-gRNA plasmids (Mettl14-KD#1 and Mettl14-KD#2), 12 h later, cellular Ca 2+ was detected by FLUO-3 AM probe and flow cytometry. C SH-SY5Y cells were treated the same as ( A ), 12 h later, cellular Ca 2+ was observed under microscope (bar = 10 μm). D The endoplasmic reticulum and mitochondria of neurons in SN of Ctrl and Mettl14-cKO mice were observed under a transmission electron microscope (bar = 1 μm left or 500 nm right). E , F The distance between ER and outer mitochondrial membrane (OMM) and the number of ER-Mito contacts per 10 μm Mitochondrial membrane surface were analyzed ( n = 4). G SH-SY5Y cells were treated the same as ( A ), 12 or 24 h later, ER stress marker proteins GRP78, ATF4 and CHOP expression were detected by WB assay. H – J Quantification of WB. All data are presented as mean ± SD, ns indicated no significant, * P < 0.05, ** P < 0.01 compared to NC.

    Article Snippet: Cell membrane permeable calcium fluorescent probe Fluo-3 AM (40703ES50) was purchased from Yeasen Biotechnology.

    Techniques: Transfection, Plasmid Preparation, Flow Cytometry, Microscopy, Transmission Assay, Membrane, Marker, Expressing

    A , B SH-SY5Y cells were transfected with pCRIPERV2-gRNA#1 plasmid (Mettl14-KD) or pCMV-Atp2a3-cDNA coding plasmid (Atp2a3-OE), combined with their empty vectors (Control: cells were transfected with empty pCRIPERV2 and empty pCMV vectors; Mettl14-KD: cells were transfected with pCRIPERV2-gRNA#1 and empty pCMV vector; KD&Atp2a3-OE: cells were transfected with pCRIPERV2-gRNA#1 and pCMV-Atp2a3-cDNA coding plasmid). Twelve hours later, cellular Ca 2+ was detected by FLUO-3 AM probe and flow cytometry. C SH-SY5Y cells were treated the same as ( A ), 12 h later, ER stress marker proteins GRP78, ATF4 and CHOP expression were detected by WB assay. D – F Quantification of WB. G , H SH-SY5Y cells were treated the same as ( A ), 12 h later, cells were treated DCFH-DA for 15 min and the cellular ROS was detected by flowcytometry. The mean DCF fluorescence intensity were quantified as relative DCFH-DA DCFH-DA level. I SH-SY5Y cells were treated the same as ( A ), 24 h later, relative cell number was quantified by cell counting assay. All data are presented as mean ± SD. * P < 0.05, ** P < 0.01 compared to Control; ## P < 0.01 compared to Mettl14-KD.

    Journal: NPJ Parkinson's Disease

    Article Title: Loss of METTL14 in dopaminergic neurons disrupts ER homeostasis via m6A-dependent regulation of Atp2a3 mRNA: Implications for Parkinson’s Disease

    doi: 10.1038/s41531-026-01318-7

    Figure Lengend Snippet: A , B SH-SY5Y cells were transfected with pCRIPERV2-gRNA#1 plasmid (Mettl14-KD) or pCMV-Atp2a3-cDNA coding plasmid (Atp2a3-OE), combined with their empty vectors (Control: cells were transfected with empty pCRIPERV2 and empty pCMV vectors; Mettl14-KD: cells were transfected with pCRIPERV2-gRNA#1 and empty pCMV vector; KD&Atp2a3-OE: cells were transfected with pCRIPERV2-gRNA#1 and pCMV-Atp2a3-cDNA coding plasmid). Twelve hours later, cellular Ca 2+ was detected by FLUO-3 AM probe and flow cytometry. C SH-SY5Y cells were treated the same as ( A ), 12 h later, ER stress marker proteins GRP78, ATF4 and CHOP expression were detected by WB assay. D – F Quantification of WB. G , H SH-SY5Y cells were treated the same as ( A ), 12 h later, cells were treated DCFH-DA for 15 min and the cellular ROS was detected by flowcytometry. The mean DCF fluorescence intensity were quantified as relative DCFH-DA DCFH-DA level. I SH-SY5Y cells were treated the same as ( A ), 24 h later, relative cell number was quantified by cell counting assay. All data are presented as mean ± SD. * P < 0.05, ** P < 0.01 compared to Control; ## P < 0.01 compared to Mettl14-KD.

    Article Snippet: Cell membrane permeable calcium fluorescent probe Fluo-3 AM (40703ES50) was purchased from Yeasen Biotechnology.

    Techniques: Transfection, Plasmid Preparation, Control, Flow Cytometry, Marker, Expressing, Fluorescence, Cell Counting